dna clean & concentrator-25 (Zymo Research)
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dna clean & concentrator-25
Dna Clean & Concentrator 25, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1044 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+concentrator/DNA+Clean+%26+Concentrator-25/custom%40d4033%4042730989
Average 99 stars, based on 1044 article reviews
Dna Clean & Concentrator 25, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1044 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+concentrator/DNA+Clean+%26+Concentrator-25/custom%40d4033%4042730989
Average 99 stars, based on 1044 article reviews
dna clean & concentrator-25 - by Bioz Stars,
2026-09
99/100 stars
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Purification:Article Title: Repurposed hnRNPC binds mature mRNAs and safeguards the mitotic transcriptome Article Snippet: The product was size-selected using Pippin Prep and a 3% agarose cassette, and a second PCR was performed using Illumina-indexed primers. .. Final libraries were purified (Zymo Article Title: High-throughput screening reveals mechanisms of environmental control of germination in a fungal thermophile. Article Snippet: Unique barcode regions were amplified using Q5 High-Fidelity DNA Polymerase with GC Enhancer (NEB M0491S) and primers target ing constant priming sites flanking each barcode, yielding a ~185 bp Illumina-ready amplicon (53). .. Amplicons were purified using Zymo Plasmid Preparation:Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability Article Snippet: .. Plasmid templates (see Supporting Information : Table S1 for all sequences) All the plasmid constructs used in this study were cloned in pCS2+ vector which was generously gifted by Antonio Giraldez lab . Nuclease-free water (Sigma-Aldrich, Cat. No. W4502-1L) 1 M TRIS (Thermo Fisher Scientific, Cat. No. 15567027) KAPA HiFi HotStart Polymerase Readymix (Roche, Cat. No. 07958927001) Oligos (see Supporting Information : Table S1) ordered from Sigma or IDT with standard desalting Oligo pools from GenScript and Twist Bioscience Thermal cycler (BIO-RAD, Model No. S1000) Construct:Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability Article Snippet: .. Plasmid templates (see Supporting Information : Table S1 for all sequences) All the plasmid constructs used in this study were cloned in pCS2+ vector which was generously gifted by Antonio Giraldez lab . Nuclease-free water (Sigma-Aldrich, Cat. No. W4502-1L) 1 M TRIS (Thermo Fisher Scientific, Cat. No. 15567027) KAPA HiFi HotStart Polymerase Readymix (Roche, Cat. No. 07958927001) Oligos (see Supporting Information : Table S1) ordered from Sigma or IDT with standard desalting Oligo pools from GenScript and Twist Bioscience Thermal cycler (BIO-RAD, Model No. S1000) Clone Assay:Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability Article Snippet: .. Plasmid templates (see Supporting Information : Table S1 for all sequences) All the plasmid constructs used in this study were cloned in pCS2+ vector which was generously gifted by Antonio Giraldez lab . Nuclease-free water (Sigma-Aldrich, Cat. No. W4502-1L) 1 M TRIS (Thermo Fisher Scientific, Cat. No. 15567027) KAPA HiFi HotStart Polymerase Readymix (Roche, Cat. No. 07958927001) Oligos (see Supporting Information : Table S1) ordered from Sigma or IDT with standard desalting Oligo pools from GenScript and Twist Bioscience Thermal cycler (BIO-RAD, Model No. S1000) Agarose Gel Electrophoresis:Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability Article Snippet: .. Plasmid templates (see Supporting Information : Table S1 for all sequences) All the plasmid constructs used in this study were cloned in pCS2+ vector which was generously gifted by Antonio Giraldez lab . Nuclease-free water (Sigma-Aldrich, Cat. No. W4502-1L) 1 M TRIS (Thermo Fisher Scientific, Cat. No. 15567027) KAPA HiFi HotStart Polymerase Readymix (Roche, Cat. No. 07958927001) Oligos (see Supporting Information : Table S1) ordered from Sigma or IDT with standard desalting Oligo pools from GenScript and Twist Bioscience Thermal cycler (BIO-RAD, Model No. S1000) Polymerase Chain Reaction:Article Title: Exosomal microRNAs from Alveolar Macrophages Reveal a Protective Role of the Lung Microbiome Against Oncogenic Signaling During PAH Exposure Article Snippet: To prepare the library, the NEBNext small RNA sample library preparation kit (New England BioLabs, Ipswich, MA, USA) was used with ~5 ng of total RNA determined by the Bioanalyzer RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA) in a 5 μL solution as input, following the manufacturer’s protocol except for a modification of library size selection to increase small RNA detection sensitivity and specificity. .. After 15 cycles of PCR for indexing and library enrichment, an equal volume of 10 μL PCR mix (library without size selection) per sample together with the same volume of the negative control were pooled, followed by DNA cleanup using Article Title: Retracing and rewriting the evolutionary trajectories of mammalian developmental enhancers Article Snippet: .. All PCR steps were cleaned up with Size Selection:Article Title: Exosomal microRNAs from Alveolar Macrophages Reveal a Protective Role of the Lung Microbiome Against Oncogenic Signaling During PAH Exposure Article Snippet: To prepare the library, the NEBNext small RNA sample library preparation kit (New England BioLabs, Ipswich, MA, USA) was used with ~5 ng of total RNA determined by the Bioanalyzer RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA) in a 5 μL solution as input, following the manufacturer’s protocol except for a modification of library size selection to increase small RNA detection sensitivity and specificity. .. After 15 cycles of PCR for indexing and library enrichment, an equal volume of 10 μL PCR mix (library without size selection) per sample together with the same volume of the negative control were pooled, followed by DNA cleanup using Article Title: Methods for the amplification of bisulfite-treated DNA Article Snippet: .. The size fractionation of the DNA fragments may be performed by methods known in the art such as gel fractionation, size exclusion chromatography, and through the use of commercially available kits such as EpiNextTM DNA Size Selection Kit (EpiGentek) and Select-a-Size Negative Control:Article Title: Exosomal microRNAs from Alveolar Macrophages Reveal a Protective Role of the Lung Microbiome Against Oncogenic Signaling During PAH Exposure Article Snippet: To prepare the library, the NEBNext small RNA sample library preparation kit (New England BioLabs, Ipswich, MA, USA) was used with ~5 ng of total RNA determined by the Bioanalyzer RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA) in a 5 μL solution as input, following the manufacturer’s protocol except for a modification of library size selection to increase small RNA detection sensitivity and specificity. .. After 15 cycles of PCR for indexing and library enrichment, an equal volume of 10 μL PCR mix (library without size selection) per sample together with the same volume of the negative control were pooled, followed by DNA cleanup using other:Article Title: FoTO1 orchestrates Taxol biosynthesis through catalytic and non-catalytic mechanisms Article Snippet: Proteins were tagged for Co-IP, immunoblot, TurboID, and BiFC by amplifying genes from plasmids or Agrobacterium glycerol stocks using PCR (PrimeStar, Takara Bio R045B, primers in Supplementary Table 3). Spectrophotometry:Article Title: Retracing and rewriting the evolutionary trajectories of mammalian developmental enhancers Article Snippet: .. All PCR steps were cleaned up with Fractionation:Article Title: Methods for the amplification of bisulfite-treated DNA Article Snippet: .. The size fractionation of the DNA fragments may be performed by methods known in the art such as gel fractionation, size exclusion chromatography, and through the use of commercially available kits such as EpiNextTM DNA Size Selection Kit (EpiGentek) and Select-a-Size Size-exclusion Chromatography:Article Title: Methods for the amplification of bisulfite-treated DNA Article Snippet: .. The size fractionation of the DNA fragments may be performed by methods known in the art such as gel fractionation, size exclusion chromatography, and through the use of commercially available kits such as EpiNextTM DNA Size Selection Kit (EpiGentek) and Select-a-Size |